Fig. 4: CD24 forms complexes with EGFR.
From: CD24: a marker of granulosa cell subpopulation and a mediator of ovulation
a Immunofluorescence staining for Cd24a in a mouse early-growing follicle (left), antral follicle (middle) and corpus luteum (right). Χ200. b Co-immunoprecipitation of EGFR by CD24 was determined. KGN cells (up) or COV434 cells (down) were immunoprecipitated with anti-CD24 antibody, followed by western blotting assays for EGFR. The second panel shows β-actin bands in samples of input and immunoprecipitation. Representative micrographs of KGN (c) and COV434 (d) cells stained for CD24 (green) and EGFR expression (red) by immunofluorescence. The arrow shows colocalization of CD24 and EGFR. Scale bar, 50 μm. n = 3 for all experiments
