Fig. 3: Kidney damage following AOLT was attenuated by inhibiting Cx43 function in vivo. | Cell Death & Disease

Fig. 3: Kidney damage following AOLT was attenuated by inhibiting Cx43 function in vivo.

From: Inhibition of gap junction composed of Cx43 prevents against acute kidney injury following liver transplantation

Fig. 3

a–c The level of Cx43 mRNA (qPCR) and Cx43 expression alternation (immunohistochemical staining and western blotting; original magnification ×200) in rat kidneys at different reperfusion time points after AOLT. n = 8, *P < 0.05 vs sham; #P < 0.05 vs 8 h after reperfusion. d “Scrape-and-load” assay was used to evaluate functional Cx43 in kidney tissue. Rats were treated with heptanol (0.1 mg/kg) or the corresponding solvent, DMSO for 1 h. The function of Cx43 was demonstrated by the spread of GJ-permeable Lucifer yellow. Rhodamine was impermeable as a negative control. DMSO had no effects on the results (data not shown), n = 6. e, f Remote kidney damage at 8 h after reperfusion, when rats were exposed to heptanol (0.1 mg/kg) or DMSO for 1 h before AOLT (H&E staining; original magnification ×200 ). DMSO had no effects on the results (data not shown), n = 8. g Changes in Cr and BUN at 8 h after reperfusion, when rats were exposed to heptanol (0.1 mg/kg) or DMSO for 1 h before AOLT. DMSO had no effects on the results (data not shown). n = 8, *P < 0.05 vs sham; #P < 0.05 vs the AOLT group

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