Fig. 2: Lung cancer-derived EVs induced epithelial cell proliferation through miR-19b and miR-92a. | Cell Death & Disease

Fig. 2: Lung cancer-derived EVs induced epithelial cell proliferation through miR-19b and miR-92a.

From: c-Myc shuttled by tumour-derived extracellular vesicles promotes lung bronchial cell proliferation through miR-19b and miR-92a

Fig. 2

a Evaluation of HBEC-KRASV12high cell viability (left; Trypan blue cell count, n = 3) and proliferation (right; CFSE analysis, n = 3) after 72 h of treatment with A549 cell- or LT73 cell-derived EVs or the corresponding EV-depleted CM. Untreated cells (NT) were used as a negative control. b 3D proliferation assay using VitroGel. Images show the colony formation ability of HBEC-KRASV12high cells treated as mentioned above. c Graphs show cells in the G2/M phase after EV treatment (n = 5). d, e Relative miR-19b (d) and miR-92a (e) expression levels in HBEC-KRASV12high cells after 24 h of the indicated treatment compared to untreated cells (n = 5). f pri-miR-92a expression levels in untreated, EV-depleted CM- and EV-treated HBEC-KRASV12high cells. B2m was used as a housekeeping control (n = 5). Data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01

Back to article page