Fig. 4: CCL2 induces expression of MCPIP1 in Mφs. | Cell Death & Disease

Fig. 4: CCL2 induces expression of MCPIP1 in Mφs.

From: CCL2 promotes macrophages-associated chemoresistance via MCPIP1 dual catalytic activities in multiple myeloma

Fig. 4

a Mφs generated from PBMCs of two independent healthy donors (Mφs-1 and Mφs-2) were treated with rhCCL2 (50 ng/ml) for 24 h. RNA was extracted for RNA sequencing. Heat maps illustrate the log2-fold change of some immune-related genes. b Mφs were exposed to rhCCL2 (50 ng/mL) for the indicated times, followed by RT-PCR and Western blotting c to determine expression of MCPIP1. d Mφs were treated with rhCCL2 (50 ng/mL) for 24 h. Immunofluorescence was performed to detect expression of MCPIP1 in Mφs. Scale bar, 50 μm. e, f Mφs were treated with RPMI-8226 CM for 24 h with or without αCCL2. qRT-PCR e and Western blotting f were performed to detect MCPIP1 expression. Data are presented as means ± SD of at least three independent experiments. *P < 0.05; **P < 0.01, ***P < 0.001

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