Fig. 7: IF-staining of L1ORF1p in the oocytes retained in Casp9−/− ovaries at 18.5 dpc. | Cell Death & Disease

Fig. 7: IF-staining of L1ORF1p in the oocytes retained in Casp9−/− ovaries at 18.5 dpc.

From: Interplay between Caspase 9 and X-linked Inhibitor of Apoptosis Protein (XIAP) in the oocyte elimination during fetal mouse development

Fig. 7

A IF-staining for TRA98 (green) and L1ORF1p (red) with DAPI nuclear staining (blue) in the microspread ovarian cells without hypotonic treatment. Scale bar, 25 μm. The area indicated with a white box is shown at ×4 magnification on the right panel, in the order of merged, DAPI alone, TRA98 alone, and L1ORF1p alone. a L1ORF1p staining in the oocyte cytoplasm. b No L1ORF1p staining in an oocyte harboring a pyknotic nucleus. c Intense L1ORF1p staining in a pyknotic nucleus. d No L1ORF1p staining in the entire oocyte. B The percentage of oocytes with L1ORF1p IF-staining in either the nucleus or cytoplasm. Data are shown as mean ± SEM (n ≧ 3). *P < 0.05, statistical significance by t-test

Back to article page