Fig. 2: TXA2 levels are elevated in HCC827GR cells and the COX2-TXA2 signaling pathway modulates MET transcription through AP-1.
From: Targeting the COX2/MET/TOPK signaling axis induces apoptosis in gefitinib-resistant NSCLC cells
a The levels of PGs were measured in the supernatants from HCC827 and HCC827GR cells. b Measurement of TXA2 and PGE2 in supernatants from shCOX2 HCC827GR cells (left), HCC827 cells stably expressing COX2 (middle), or HCC827GR cells treated with celecoxib for 48 h at different doses (right). c HCC827 cells were treated with U46619 for 48 h at different doses (left); HCC827GR cells were treated with different doses of Seratrodast (middle) or Ozarel HCl (right) for 48 h. d HCC827 cells stably expressing COX2 (left) or HCC827GR cells (right) were treated with SR11302 for 48 h. e The stable shCOX2 HCC827GR cells were treated with gefitinib (1 μΜ) for 6 h (left); HCC827GR cells were treated with different doses of celecoxib for 48 h (right). Those samples were resolved by SDS-PAGE and visualized by western blotting. Data are representatives of results from triplicate experiments. *P < 0.05, **P < 0.01, ***P < 0.001
