Fig. 2: Tet regulates hPDLSC-mediated immunomodulation. | Cell Death & Disease

Fig. 2: Tet regulates hPDLSC-mediated immunomodulation.

From: Inhibition of Tet1- and Tet2-mediated DNA demethylation promotes immunomodulation of periodontal ligament stem cells

Fig. 2

a Western blot analyzed the efficiency of Tet1 and Tet2 small interfering RNA (siRNA) knockdown in hPDLSCs. b BrdU labeling assay was performed to show elevated proliferation rates of Tet1 and Tet2 siRNA-treated hPDLSCs. Scale bar, 50 µm. c Flow cytometry was used to analyze the expression of CD105, CD90, CD73, CD34, and CD45 in control and Tet1 and Tet2 siRNA-treated hPDLSCs. d, e In vitro coculture showed a significantly increased capacity of Tet1 and Tet2 siRNA-treated hPDLSCs to induce T cell apoptosis (AnnexinV+7AAD+) of T cells. ***P < 0.001, **p < 0.01, and *p < 0.05; p values were calculated using two-tailed Student’s t test (mean ± SD)

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