Fig. 3: Inhibition of Tet1 and Tet2 enhances PDLSC-mediated amelioration of disease phenotype in colitis mice. | Cell Death & Disease

Fig. 3: Inhibition of Tet1 and Tet2 enhances PDLSC-mediated amelioration of disease phenotype in colitis mice.

From: Inhibition of Tet1- and Tet2-mediated DNA demethylation promotes immunomodulation of periodontal ligament stem cells

Fig. 3

a Schema showing PDLSC transplantation for treating colitis mice. b–e Knockdown of Tet1 and Tet2 by siRNA treatment elevated the immunomodulatory capacity of PDLSCs, as assessed by amelioration of the reduced body weight (b), a decreased disease activity index (DAI) (c), and alleviation of the colitis histologic activity index (HAI). Scale bar in d, 100 µm. f Flow cytometry analysis showed that the Treg level significantly decreased in colitis mice compared to control littermates. After PDLSC treatment, the Treg level was significantly elevated, and the Tet1 and Tet2 siRNA-treated PDLSC group showed a higher Treg level than the group with control PDLSCs. ***P < 0.001, **p < 0.01, *p < 0.05; p values were calculated using two-tailed Student’s t test (mean ± SD)

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