Fig. 5: FOXO3a is a downstream target of miR-223 in HCC cells. | Cell Death & Disease

Fig. 5: FOXO3a is a downstream target of miR-223 in HCC cells.

From: miR-223 overexpression inhibits doxorubicin-induced autophagy by targeting FOXO3a and reverses chemoresistance in hepatocellular carcinoma cells

Fig. 5

a Prediction of miR-223 targets in TargetScan. Predicted consequential pairing of target region in the FOXO3a 3’-UTR (top) and has-miR-223-3p (bottom). b, c FOXO3a protein expression in control HCC cells and HCC cells transfected with NC mimic, miR-223 mimic, NC inhibitor, or miR-223 inhibitor for 48 h. d, e FOXO3a mRNA expression in control HCC cells and HCC cells transfected with NC mimic, miR-223 mimic, NC inhibitor, or miR-223 inhibitor for 48 h (*p < 0.05, **p < 0.01, miR-223 mimic vs. NC mimic or miR-223 inhibitor vs. NC inhibitor). f Effect of NC mimic or miR-223 mimic on luciferase activity in 293T cells transfected with either the wild type or mutant FOXO3a 3’-UTR psiCHECK2 plasmid (*p < 0.05, miR-223 mimic vs. NC mimic)

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