Fig. 3: Exogenous rCav-1 promotes CSC phenotypes.
From: The paracrine induction of prostate cancer progression by caveolin-1
a Prostate sphere formation of WT LNCaP under the influence of Cav-1-expressing cells was determined after plating LNCaP WT (500 cells) onto the bottom of an ultralow attachment 24-well plate and either LNCaP Vc or Cav1OE (5 × 104 cells) onto a Transwell (3.0-µm polycarbonate). Number of spheres was determined 14 days after co-culture. Scale bar = 50 μm. b Upper panel: prostate sphere formation of LNCaP WT incubated with rCav-1 (from 0 to 100 ng/ml) was determined after 14 days. Lower panel: rCav-1 uptake by WT LNCaP was analyzed by western blot. Scale bar = 100 μm. c Upper panel: prostate sphere formation of Du145 shCav1 (low Cav-1 expression) incubated with rCav-1 (from 0 to 150 ng/ml) was determined after 14 days. Lower panel: rCav-1 uptake by Du145 shCav1was analyzed by western blot. Scale bar = 100 μm. d The gene expression profile of Yamanaka factors gene (Left panel) and NED-related genes (Right panel) was examined 48h post treatment. Scale bar = 50 μm. All data were shown as mean ± SD (n > 10), *P < 0.5, **P < 0.01, ***P < 0.001
