Fig. 6: CRC cell-derived exosomal RPPH1 induces macrophages M2 polarization. | Cell Death & Disease

Fig. 6: CRC cell-derived exosomal RPPH1 induces macrophages M2 polarization.

From: LncRNA RPPH1 promotes colorectal cancer metastasis by interacting with TUBB3 and by promoting exosomes-mediated macrophage M2 polarization

Fig. 6

a, b Exosomes isolated from supernatants of CRC cells via ultracentrifugation were detected by transmission electron microscopy and Particle Metrix. Scale bar = 100 μm. c WB analysis of exosomes markers TSG101 and CD9. Exosomes extracts were treated with Triton X-100 + proteinase K or proteinase K alone. d PAGE analysis of RPPH1 levels in exosomes after RT-PCR treated with RNase (2 mg/ml) alone or combined with Triton X-100 (0.1%) for 15 min. e, f Confocal microscopy and flow cytometric analysis of the internalization of PKH26-labeled exosomes in MDMs. Scale bar = 10 μm. g Flow cytometric analysis of the expressions of CD206/HLA-DR in macrophages treated with different concentrations of exosomes isolated from supernatants of CRC cells. Numerical values denote the relative fluorescence intensity. h PAGE analysis of RPPH1 levels in exosomes isolated from supernatants of stable RPPH1 overexpression or knockdown cells. i Confocal microscopy of the macrophages treated with exosomes with different RPPH1 levels. Scale bar = 10 μm. j Flow cytometric analysis of the expressions of CD206/HLA-DR in macrophages treated with exosomes with different RPPH1 levels. Numerical values denote the relative fluorescence intensity

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