Fig. 2: RIP3ko exacerbates striatal astrogliosis and replenishes GDNF protein levels in MPTP-injected RIP3ko mice when compared to Wt mice.

a Representative images of Iba1-positive microglia in the striatum of control- and MPTP-injected mice from both genotypes and respective quantification. Scale bar, 100 μm. b Representative images of GFAP-positive astrocytes in the striatum of control- and MPTP-injected mice from both genotypes and respective quantification. Scale bar, 100 μm. c Representative WB of the SN of control– and MPTP-injected Wt and RIP3ko mice and respective densitometric analysis. Values represent mean ± SEM of 6-7 mice per group. *p < 0.05 from control Wt mice and ^§p < 0.05 from MPTP-injected Wt mice. d Primary mixed glial cultures were exposed to 100 ng/ml LPS, 50 ng/ml TNF-α, 100 U/ml IFNγ or TNF-α + IFNγ for 24 h and pro-inflammatory gene expression was determined by qRT-PCR. Values represent mean ± SEM of three independent experiments. ^†p < 0.05 from respective Wt group