Fig. 6: Increase of mtROS in combination with cisplatin enhances cell death in resistant cells. | Cell Death & Disease

Fig. 6: Increase of mtROS in combination with cisplatin enhances cell death in resistant cells.

From: Direct impact of cisplatin on mitochondria induces ROS production that dictates cell fate of ovarian cancer cells

Fig. 6

a, b Flow cytometric analysis of the cisplatin resistant cell line OVCAR-8 after co-treatment with 100 µM Genipin and 10 µM cisplatin for 48 h. a Genipin increases cisplatin mediated mtROS induction (MitoSOX red). b Viability (Annexin V negative/PI negative) of OVCAR-8 cells is reduced by co-incubation with Genipin and cisplatin. c Knock-down of UCP2 sensitizes OVCAR-8 to cisplatin induced apoptosis. d, e Flow cytometric analysis of OVCAR-8 cells after co-treatment with 2 mM ascorbic acid and 10 µM cisplatin for 48 h. d Ascorbic acid increases cisplatin mediated mtROS induction (MitoSOX red). e Viability (Annexin V negative/PI negative) of OVCAR-8 cells is reduced by co-incubation with ascorbic acid and cisplatin. f Flow cytometric analysis reveals reduced viability (Annexin V negative/PI negative) of OVCAR-8 cells after co-treatment with 300 µM H2O2 and 10 µM cisplatin for 48 h. Data represent means ± SD from at least three independent experiments

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