Fig. 2: S3I-201 reduces accumulation of collagen and expression of pro-fibrotic signaling molecules in kidney tissue of diabetic mice. | Cell Death & Disease

Fig. 2: S3I-201 reduces accumulation of collagen and expression of pro-fibrotic signaling molecules in kidney tissue of diabetic mice.

From: Inhibition of STAT3 in tubular epithelial cells prevents kidney fibrosis and nephropathy in STZ-induced diabetic mice

Fig. 2

The animal treatment and groups were described in Fig. 1 and Methods section; n = 7 in each group. a Histological depots of collagen fibers (indicated by arrows) as visualized with sirius red staining; shown are representative images from seven mice per group, 400× amplification; b Quantification of collagen accumulation from the sirius red stained images using Image J software; values reported as normalized to Ctrl; c Kidney tissue collagen IV, TGF-β1, ACE, and AT1 mRNA was determined by quantitative RT-PCR; values normalized to the house-keeping gene, β-actin. d Representative Western blot analysis of TGF-β, ACE, AT1, and VEGF in duplicates, GAPDH as loading control. e Corresponding densitometric analysis of blots in e, calculating from densitometric values of repective bands normalized to loading control GAPDH. For b, c, and e, data are presented as means ± SEM; (n = 7; #p < 0.05, ##p < 0.01 versus Ctrl; *p < 0.05, **p < 0.01 versus T1DM group.)

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