Fig. 2: Sub-mitochondrial localization of wild type and mutant α-syn was analyzed in undifferentiated. | Cell Death & Disease

Fig. 2: Sub-mitochondrial localization of wild type and mutant α-syn was analyzed in undifferentiated.

From: A split-GFP tool reveals differences in the sub-mitochondrial distribution of wt and mutant alpha-synuclein

Fig. 2

a and differentiated b BE(2)-M17 dopaminergic-like cells by the co-expression of the OMM, IMS and mitochondrial matrix targeted GFP1–10 non-fluorescent moiety and of the WT, A53T and A30P α-synS11. Confocal images were acquired at 488 and 594 nm excitation wavelength. Transfected cells were incubated with an anti α-syn primary antibody and stained with an Alexa 633 conjugated secondary antibody. Complementation of the GFP probes was revealed by fluorescent acquisition at 488 nm excitation wavelength. Scale bar is 20 μm

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