Fig. 4: Quantification of wild type and mutant α-syn distribution at the OMM and IMS. Expression of the OMM. | Cell Death & Disease

Fig. 4: Quantification of wild type and mutant α-syn distribution at the OMM and IMS. Expression of the OMM.

From: A split-GFP tool reveals differences in the sub-mitochondrial distribution of wt and mutant alpha-synuclein

Fig. 4

a and IMS c targeted GFP1-10 non-fluorescent moiety and of the WT, A53T and A30P α-synS11. Transfected cells were incubated with an anti α-syn primary antibody and stained with an Alexa 633 conjugated secondary antibody. Complementation of the GFP probes was revealed by fluorescent acquisition at 488 nm excitation wavelength a, c. The corrected total cell fluorescence (CTCF) was quantified as described in the ‘Materials and Methods’ section and reported as normalized values with respect to WT α-syn b, d (****p < 0.0001, ***p < 0.001, **p < 0.01). One-way ANOVA retrieved a p value of 0.0001 for b and d. Dunnett’s multiple comparison test retrieved a statistically significant difference between WT and A53T p < 0.001 and WT and A30P p < 0.01 b and between WT and A53T p < 0.001 and WT and A30P p < 0.001. Quantification of CTCF has been done on at least 29 cells from at least three independent experiments for each transfection condition. Scale bar is 20 μm

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