Fig. 1: HUVECs-derived sEVs protected neurons from OGD injury in culture.

a The coculture of HUVECs and neurons in a transwell system. Primary neurons were stained with MAP-2 and counter-stained with Hoechst. b EVs were precipitated from equal amounts of supernatants of the coculture and evaluated by Western blotting of the EV markers. c, d TUNEL staining of neurons in the coculture assay in the presence of DMSO or GW4869. Apoptotic neurons were shown (folds compared with the control) d. Bars = means ± s.e.m., n = 5. **P < 0.01.