Fig. 3: miR-1290 and miR-1246 in HUVECs-derived EVs exhibited protective effect on OGD-treated neurons.

a EC-derived and apoptosis-related miRNAs in literatures were screened and illustrated with a Venn plot. b The levels of 10 miRNAs in a were determined in EVs derived from HUVECs. c, d Primary neurons were treated by OGD and incubated with control oligos (NC) or mimics of miR-1246 and miR-1290. The apoptosis of neurons was evaluated by TUNEL. e Cell death was evaluated by LDH release. f The protein level of aCasp3 in neurons from different groups was measured by Western blotting. Bars = means ± s.e.m, n = 3. *P < 0.05, **P < 0.01, n.s., not significant.