Fig. 2: IL-6^−/− and UCP-1^−/− KO mice are protected from burn-induced browning and hepatic steatosis post-injury.

a, b Changes in total body (a) and fat (b) weight in IL-6^−/− burned mice and IL-6^−/− controls. c Plasma concentration of free fatty acids in IL-6^−/− burned mice and controls. d UCP1 staining in inguinal WAT of WT and IL-6^−/− burned mice and controls. e Quantitative RT-PCR analysis of browning gene UCP1 in inguinal WAT of wild type (WT) and IL-6^−/− burned mice and controls. f Liver weights normalized to body weight of WT and IL-6^−/− burned mice and controls. g Oil Red O staining for fat droplets in liver sections from WT and IL-6^−/− burned mice and controls. h Triglyceride (TG) content of livers from WT and IL-6^−/− burned mice and controls. i H&E and UCP1 staining in inguinal WAT of WT and UCP-1^−/− burned mice and controls. j Quantitative RT-PCR analysis of browning gene UCP1 in inguinal WAT of WT and UCP-1^−/− burned mice and controls. k Plasma concentration of free fatty acids in UCP-1^−/− burned mice and controls. l Oil Red O staining for fat droplets in liver sections from WT and UCP-1^−/− burned mice and controls. m Triglyceride (TG) content of livers from WT and UCP-1^−/− burned mice and controls. Data represented as mean ± SEM, p < 0.05 *significant difference WT burn vs. controls, p < 0.05 # WT burn vs. IL-6^−/− / UCP-1^−/− (n = 7, biological replicates, experiments repeated two times).