Table 1 Sequences of the study.
From: PGK1 depletion activates Nrf2 signaling to protect human osteoblasts from dexamethasone
Genes | Forward primer sequence (5′–3′), qPCR | Reverse primer sequence (5′–3′), qPCR |
|---|---|---|
PGK1 | CCGCTTTCATGTGGAGGAAGAAG | CTCTGTGAGCAGTGCCAAAAGC |
GCLC | GGAAGTGGATGTGGACACCAGA | GCTTGTAGTCAGGATGGTTTGCG |
HO1 | CCAGGCAGAGAATGCTGAGTTC | AAGACTGGGCTCTCCTTGTTGC |
NQO1 | AGGCTGGTTTGAGCGAGTTC | ATTGAATTCGGGCGTCTGCTG |
Nrf2 | CACATCCAGTCAGAAACCAGTGG | GGAATGTCTGCGCCAAAAGCTG |
GAPDH | GTCTCCTCTGACTTCAACAGCG | ACCACCCTGTTGCTGTAGCCAA |
PGK1 sgRNA | AGCTGGACGTTAAAGGGAAG (Target DNA sequence) | PAM sequence: CGG |
PGK1 shRNA-S1 | AAGAACAACCAGATAACAAACAA | |
PGK1 shRNA-S2 | AAGGATGTTCTGTTCTTGAAGGA |
