Fig. 7: sna activates puc transcription in vivo.

a–f Light micrographs showing X-Gal staining of a puc-LacZ reporter in wing discs. Compared with the control a, overexpression of Hep^WT or Sna in the wing pouch notably upregulated puc transcription b and c. Compared with ptc-GAL4 d, the increased puc-LacZ expression triggered by Hep^WT along the A/P compartment boundary e is partially impeded in heterozygous sna mutant f. g–j Fluorescence micrographs of Drosophila third instar larval wing discs are shown. The elevated JNK phosphorylation induced by knockdown of dlg g and g′ remains unaffected by RNAi-mediated depletion of sna j and j′. LacZ and Bsk^DN expressions are used here as negative and positive controls, respectively h, h′, i and i′. See the electronic supplementary material for detailed genotypes. Scale bar: 50 μm in a–c, 20 µm in d–j.