Fig. 1: Injured podocytes re-entered the cell cycle.

a Urinary albumin/creatinine ratio in mice of the control group and adriamycin-treated group; error bars indicate the SD (n = 3). b Immuno- histochemical staining showed expression of PCNA in the glomerulus; podocytes are indicated by an arrow (magnification, ×200). c In vitro experiments showed the expression of PCNA in podocytes after adriamycin (0.25 µg/mL) treatment for the indicated time (magnification, ×200). d Schematic diagram of cell cycle detection results; the diagonal strip area represents the proportion of cells in S phase. e The proportion of cells in S phase in different groups; data are from at least three replicates. f–h Real-time PCR detection of cell cycle-related gene expression after adriamycin treatment; data are from at least three replicates. i Western blotting analysis of the expression of cell cycle-associated proteins and their inhibitors in podocytes treated with adriamycin (0.25 µg/mL) for the indicated time periods. j Quantification of band density relative to the β-actin control (n = 3 per group). k Immunohistochemical analysis showed that CDK4 and cyclin D1 were expressed in podocytes of adriamycin-treated mice; the arrow indicates the location of the podocyte and the magnification was ×400. CON control, ADR Adriamycin, DAPI 4′,6-diamidino-2-phenylindole. *P < 0.05 vs. control group.