Fig. 2: Cell cycle re-entry sensitised podocytes to dedifferentiation. | Cell Death & Disease

Fig. 2: Cell cycle re-entry sensitised podocytes to dedifferentiation.

From: Yes-associated protein regulates podocyte cell cycle re-entry and dedifferentiation in adriamycin-induced nephropathy

Fig. 2

a Immunofluorescence staining for the detection of snail2 and desmin expression in mouse kidneys; the arrow indicates co-staining of snail 2/desmin (red) and podocalyxin (green) in podocytes (magnification, ×400). b Immunohistochemical staining showed that some CDK4-positive podocytes in adriamycin-treated mice began to express desmin. The arrow indicates podocytes expressing CDK4 and desmin simultaneously (magnification, ×400). c, d mRNA expression of dedifferentiation-related genes and podocyte markers examined by real-time PCR after adriamycin treatment for the indicated time periods. e The effect of adriamycin on the expression of snail2 and desmin was determined by western blotting analyses. f Immunoblotting quantification of (E). g Western blotting analysis of podocyte marker protein expression after adriamycin (0.25 µg/mL) treatment for the indicated times. h Immunoblotting quantification of (G). Error bars are ± SD. (n = 3 per group). *P < 0.05 vs. control group.

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