Fig. 3: Spatial evolution of BMSCs during the process of dissemination. | Cell Death & Disease

Fig. 3: Spatial evolution of BMSCs during the process of dissemination.

From: Cancer-educated mesenchymal stem cells promote the survival of cancer cells at primary and distant metastatic sites via the expansion of bone marrow-derived-PMN-MDSCs

Fig. 3

GFP-BMSCs from the primary inoculation cancer site and bone marrow were collected by flow cytometer sorting technology. The cellular RNA from BMSCs was subjected to RNA-sequencing. a Principal component analysis plot. b Hierarchical clustering analysis of gene expression. Clustering was performed on differentially expressed genes at FDR < 0.05 from nine samples of BMSCs. Columns represent individual samples and rows represent each gene. Each cell in the matrix represents the expression level of a gene feature in an individual sample. Red and green in cells reflect high and low expression levels, respectively, as indicated in the scale bar (log2-transformed scale). c Hierarchical clustering analysis on differentially expressed genes related to selected functions. Columns represent individual samples and rows represent each gene. d Expression of genes enriched in the osteoclast differentiation and immunosuppressive pathway quantified by RNA-Seq. FPKM for selected gene transcripts obtained by RNA-Seq. e The expression of genes was validated by real-time PCR. Data were analyzed with Student’s t-test, *p < 0.05; **p < 0.01; ***p < 0.001.

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