Fig. 4: MiR-1205 directly targets E2F1. | Cell Death & Disease

Fig. 4: MiR-1205 directly targets E2F1.

From: Reciprocal regulation of miR-1205 and E2F1 modulates progression of laryngeal squamous cell carcinoma

Fig. 4

a Western blot analysis of E2F1 protein expressions in the indicated cells. b A schematic diagram of the reporter constructs showed the wild type (Wt) and mutant (Mut) sequences of the miR-1205-binding sites within human E2F1 3′-UTR. c Luciferase activity of reporters with E2F1 Wt or Mut 3′-UTR in the indicated cells. d, e Western blot analysis and quantification of E2F1 protein expression in the tumors of the indicated cells. GAPDH was the loading control. Data are presented as mean ± s.d. Student’s t-test was used for statistical analysis; **P < 0.01.

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