Fig. 6: E2F1 partially reverses the suppressive effects of miR-1205 on the growth, migration, and invasion of LSCC cells.

a Western blot analysis of E2F1, cyclin E, and survivin protein expressions in the indicated cells. GAPDH is the loading control. b Cell proliferation of the indicated cells as determined with MTT assay. Representative images and quantification of the indicated cells sphere as determined with sphere formation assay. Scale bar, 200 μm c, cells migration as determined with wound healing assay d and cells invasion as determined with transwell assay e. Data are presented as mean ± SD. Student’s t-test was used for statistical analysis; *P < 0.05; **P < 0.01.