Fig. 4: Ibrutinib-induced FOXO3a upregulates PTEN that antagonizes AKT to induce apoptosis. | Cell Death & Disease

Fig. 4: Ibrutinib-induced FOXO3a upregulates PTEN that antagonizes AKT to induce apoptosis.

From: Resistance to BTK inhibition by ibrutinib can be overcome by preventing FOXO3a nuclear export and PI3K/AKT activation in B-cell lymphoid malignancies

Fig. 4

a RIVA cells were transfected with siFOXO3a and siControl and treated with ibrutinib (10 µM) for 24 h. Expression levels of FOXO3a, PTEN, pFOXO3aSer253, BIM, and cleaved Caspase 3 were determined by immunoblotting with the indicated antibodies. GAPDH was used as a loading control. b Cell viability was determined by Annexin V-PI staining. Control cells were treated with DMSO. (*p < 0.05, **p < 0.01). SD is indicated as error bars (N = 3). c MEC-1 cells were transfected with siPTEN and siControl and treated with ibrutinib (10 µM) for 24 h. Expression levels of PTEN, pAKT, AKT, pFOXO3aSer253, FOXO3a, and cleaved Caspase 3 were determined by immunoblotting with the indicated antibodies. GAPDH was used as a loading control. d Cell viability was determined by Annexin V-PI staining. Control cells were treated with DMSO. (*p < 0.05). SD is indicated as error bars (N = 3).

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