Fig. 2: Evi1 prevents LC maturation and promotes LSC properties, and its effects are enhanced by atRA. | Cell Death & Disease

Fig. 2: Evi1 prevents LC maturation and promotes LSC properties, and its effects are enhanced by atRA.

From: All-trans retinoic acid enhances, and a pan-RAR antagonist counteracts, the stem cell promoting activity of EVI1 in acute myeloid leukemia

Fig. 2

a Schematic of experimental design. FC, flow cytometry. b Kaplan–Meier plot of mice transplanted with shCtrl or shEvi1-transduced LCLSK_MA9 (250,000 cells/mouse). n = 4/group; *p < 0.05; log-rank test. c Relative Evi1 mRNA levels in BM LCLSK_MA9_shCtrl and LCLSK_MA9_shEvi1 (left panel) and the corresponding LSCe (right panel). qRT-PCR; n = 3; ***p < 0.001; t-test. d–g BM cells (d–f) or BM LCs (Venus+ RFP+ cells; panel g) from terminally ill mice were treated with 1 µM atRA or solvent for 3 days. n = 3; *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant; ANOVA followed by Bonferroni's post-hoc test. d Myeloid differentiation. e Proportions of LSCe among LCs. f Proportions of quiescent LSCe (LSCe in G0). g Colony formation in methyl cellulose, presented as percent of solvent-treated LCLSK_MA9_shCtrl in each round of plating.

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