Fig. 7: EVI1 and atRA cooperatively promote quiescence in human myeloid cell lines with stem cell characteristics. | Cell Death & Disease

Fig. 7: EVI1 and atRA cooperatively promote quiescence in human myeloid cell lines with stem cell characteristics.

From: All-trans retinoic acid enhances, and a pan-RAR antagonist counteracts, the stem cell promoting activity of EVI1 in acute myeloid leukemia

Fig. 7

a, b Immunoblot analyses of HNT-34 (a) and UCSD/AML1 (b) derivative cell lines. Cells were treated with doxycycline for 2 days to induce shRNAs. Left panels, representative experiments. Right panels, quantification; n = 3; **p < 0.01; ***p < 0.001; t-test. (c–h) HNT-34 and UCSD/AML1 derivative cell lines were treated with doxycycline for 2 days; 1 µM atRA or solvent was added for another 3 days. n = 3; *p < 0.05; **p < 0.01; ***p < 0.001; ns, not significant; ANOVA followed by Bonferroni's post-hoc test. c, d Myeloid differentiation (mean fluorescence intensity (MFI) of CD11b). HNT-34 (c), UCSD/AML1 (d). e, f Relative viability (measured using metabolic activity as a proxy). HNT-34 (e), UCSD/AML1 (f). g, h Proportion of quiescent cells (cells in G0). HNT-34 (g), UCSD/AML1 (h). For all panels, control experiments performed in the absence of doxycycline are shown in Supplementary Fig. S5.

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