Fig. 1: Targeted disruption of mouse RAI16 gene.
From: Retinoid acid induced 16 deficiency aggravates colitis and colitis-associated tumorigenesis in mice
a Schematic representation of the gene targeting strategy for exon 2 of the RAI16 gene. (A section of 37 bp was deleted in RAI16 DNA, however, the whole exon 2 was skipped in RAI16 mRNA). The red “*” represents the stop codon. b PCR analysis of RAI16 DNA extracted from the tails of WT and RAI16−/− mice. c RT-PCR analysis of RAI16 mRNA extracted from the colon tissues of WT and RAI16−/− mice. d RT-PCR products were confirmed by the sequencing.
