Fig. 4: miR-26a/b regulate cytokines and target CD38.
From: MicroRNA-26a/b have protective roles in oral lichen planus
a Heat map showing alterations of cytokines and corresponding receptors expression in HOKs with activated CD4+ T cells or LPS stimulation by real-time PCR. b Real-time PCR analysis of relative IFNγ status in activated CD4+ T cells or LPS-stimulated HOKs with 36-h miR-26a/b pre-treatment. c Putative miR-26a/b binding site in the 3′UTR of CD38 mRNA. d Schematic showing binding site location in the 3′UTR of hCD38 cDNA. e Luciferase reporter assay showing miR-26a/b target binding site in the 3′UTR of hCD38 in HOKs. f–g Luciferase activity quantification in HOKs. Cells were co-transfected with Luc-CD38-3′UTR or Luc-CD38-3′UTR-Mut and miR-26a/b inhibitors as shown. *P < 0.05, **P < 0.01, ***P < 0.001 vs. corresponding control; n = 3. Ctrl control, In inhibitor, Mut mutation.
