Fig. 1: Dietary ω-6 PUFA supplementation induces autophagy but decreases antioxidant ability in vivo. | Cell Death & Disease

Fig. 1: Dietary ω-6 PUFA supplementation induces autophagy but decreases antioxidant ability in vivo.

From: ω-6 Polyunsaturated fatty acids (linoleic acid) activate both autophagy and antioxidation in a synergistic feedback loop via TOR-dependent and TOR-independent signaling pathways

Fig. 1

Activity of antioxidant enzyme (CAT, SOD, MDA, Gpx, and T-AOC) were examined in the livers of experimental fish (n = 3) (a). The mRNA expression levels of key hepatic antioxidation-related genes (Nrf2, Sod1, Sod3, CAT, and Gpx) were analyzed (n = 6) (b). Nuclear Nrf2 in the liver was examined by western blot analysis and quantitated after treatment with different diets (n = 3) (c). Macroautophagy in the liver was identified by TEM after treatment with different diets. Scale bars, 5 μm (d). The LC3-II/LC3-I ratio in the liver was examined by western blot analysis and quantitated after treatment with different diets (n = 3) (e). The FO diet group was the control group. The results are presented as the mean with SEM and were analyzed using independent t-tests (*P < 0.05).

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