Fig. 1: CircRNA and mRNA expression profiling and identification of circCTNNA1 and FOXM1 in colon cancer. | Cell Death & Disease

Fig. 1: CircRNA and mRNA expression profiling and identification of circCTNNA1 and FOXM1 in colon cancer.

From: Circular RNA circCTNNA1 promotes colorectal cancer progression by sponging miR-149-5p and regulating FOXM1 expression

Fig. 1

a, b Ten significantly upregulated and downregulated circRNAs and mRNA are demonstrated by heatmaps. c A KEGG pathway analysis showed the most enriched pathways of the differentially expressed genes. d circCTNNA1 derives from back-spliced exons 10 to 13 of CTNNA1. The back-splice junction of circCTNNA1 was identified by Sanger sequencing. e PCR analysis for circCTNNA1 and linear CTNNA1 in cDNA and gDNA. f RNase R digestion assay was conducted to evaluate the stability of circCTNNA1. g The circCTNNA1 expression in 60 pairs of colon cancer and adjacent normal tissues was measured by qRT-PCR assays. h Kaplan–Meier survival analysis showed that patients with high circCTNNA1 expression had a poorer prognosis. I, j FOXM1 expression were determined by qRT-PCR and IHC in the 60 pairs of colon cancer tissues and matched normal tissues. k RNA sequencing data from TCGA-COAD database showed that FOXM1 was significantly upregulated in the colon cancer tissues compared with normal tissues. l Pearson correlation analysis showed that FOXM1 expression were positively correlated with that of the circCTNNA1. ***p < 0.001.

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