Fig. 2: CircCTNNA1 promotes the growth, invasion, and migration of colon cancer cells in vitro.

a CircCTNNA1 expression in normal and colon cancer cell lines was measured using qRT-PCR. b, c The effects of overexpression or knockdown of circCTNNA1 in SW620 or SW480 cells were measured using qRT-PCR. d knockdown and overexpression of circCTNNA1 had no effect on the expression level of linear mRNA of CTNNA1. e, f CCK-8 and colony formation assays of colon cancer cells with circCTNNA1 overexpression or knockdown. g, h EdU incorporation assay revealed the changes of DNA synthesis after overexpression or knockdown of circCTNNA1. I, j Transwell and wound-healing assays showed that invasion and migration were significantly increased after circCTNNA1 overexpression, but inhibited after circCTNNA1 knockdown. *p < 0.05, **p < 0.01, ***p < 0.001.