Fig. 4: CircCTNNA1 functions as a sponge of miR-149-5p. | Cell Death & Disease

Fig. 4: CircCTNNA1 functions as a sponge of miR-149-5p.

From: Circular RNA circCTNNA1 promotes colorectal cancer progression by sponging miR-149-5p and regulating FOXM1 expression

Fig. 4

a Potential target miRNAs of circCTNNA1 were predicted by CircInteractome and miRanda online database. b, c CircRNA pull-down assays were performed with a specific biotin-labeled circCTNNA1 probe in SW620 and SW480 cells. d The expression levels of miR-149-5p in 60 colon cancer tissues were determined by qRT-PCR. e RNA sequencing data from TCGA-COAD database showed that miR-149-5p was underexpressed in colon cancer. f RIP assay was conducted using an AGO2 antibody, and IgG served as a negative control. g, h circCTNNA1 and miR-149-5p were both significantly enriched in cells transfected with miR-149-5p mimics. i The direct binding between circCTNNA1 and miR-149-5p was analyzed by dual-luciferase reporter assay. j FISH assay showed the co-localization of circCTNNA1 and miR-149-5p in colon cancer cells. k Correlation between the expression levels of circCTNNA1 and miR-149-5p in colon cancer tissues. Scale bar = 100 µm. *p < 0.05, **p < 0.01, ***p < 0.001.

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