Fig. 6: FOXM1 is a direct target of miR-149-5p and indirectly regulated by circCTNNA1. | Cell Death & Disease

Fig. 6: FOXM1 is a direct target of miR-149-5p and indirectly regulated by circCTNNA1.

From: Circular RNA circCTNNA1 promotes colorectal cancer progression by sponging miR-149-5p and regulating FOXM1 expression

Fig. 6

a TargetScan showed that miR-149-5p could bind to the 3′UTR of FOXM1. b The direct binding between FOXM1 3′UTR and miR-149-5p was analyzed by dual-luciferase reporter assay. c, d FOXM1 expression in normal and colon cancer cell lines were measured by western blotting and qRT-PCR. e, f The expression levels of FOXM1 were measured by western blotting after transfection of miR-149-5p mimic or inhibitor. g Pearson correlation analysis revealed the negative correlation between FOXM1 and miR-149-5p. h The protein expression levels of FOXM1 and its downstream genes affected by the knockdown or overexpression of circCTNNA1 were reversed by co-transfection of the miR-149-5p inhibitor or mimic. i Schematic diagram of the mechanism and function of circCTNNA1 in colon cancer progression. *p < 0.05, **p < 0.01, ***p < 0.001.

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