Fig. 6: Upregulation of antioxidant and proproliferative genes during heart regeneration is impaired in the injury Δ113p53M/M mutant hearts.
From: p53 isoform Δ113p53 promotes zebrafish heart regeneration by maintaining redox homeostasis
a–f Relative mRNA expression of Δ113p53 (a), gpx1a (b), sesn2 (c), hif1al2 (d), jak2a (e) and pim2 (f) in the Δ113p53+/+ and Δ113p53M/M hearts at sham and 14 dpa. The total RNA was extracted from a pool of at least 10 hearts in each group. g Western blot was performed to analyse the induction of zebrafish Hif1α in different samples as indicated. The Δ113p53+/+ and Δ113p53M/M mutant zebrafish with heart resection were treated with DPI from 3 to 7 dpa. Total protein was isolated from four hearts/treatment at 7 dpa and subjected to western blot analysis. Gapdh was used as the protein loading control. The experiments were repeated independently for at least three times with similar results. Statistical analysis was performed on relevant data using Student’s two-tailed t test in GraphPad Prism 5. The p values were represented by n.s. and asterisks. n.s., p > 0.05. *p < 0.05. **p < 0.01. ***p < 0.001.
