Fig. 2: Gain of miR-1224-5p inhibits and loss of miR-1224-5p promotes the proliferation, colony formation, migration and invasion of oesophageal cancer cells.
From: The miR-1224-5p/TNS4/EGFR axis inhibits tumour progression in oesophageal squamous cell carcinoma
a The expression of miR-1224-5p in ESCC cell lines and the human oesophageal epithelial cell line Het1A was examined by qRT-PCR. b, c The transfection efficiencies of mimic and inhibitor of miR-1224-5p in ESCC cell lines were measured by qRT-PCR. d–k CCK-8 and colony formation assays were used to analyse the proliferation ability of transfected cells. l–o The migration and invasion abilities of transfected cells were measured by transwell assay. Scale bar, 200 μm. Data are represented as the mean ± SD, and experiments were performed in triplicate; ** p < 0.01, *** p < 0.001.
