Fig. 5: EN2 promotes cell proliferation and migration of CRC via activating CCL20 signaling.
From: EN2 as an oncogene promotes tumor progression via regulating CCL20 in colorectal cancer
a The mRNA level of EN2 in SW480 cells transfected with vector, si-EN2 and si-EN2 + CCL20. b–e Cell proliferation and migration detected through CCK8 assay (b), Edu assay (c), transwell assay (d), and wound healing test (e) of SW480 cells transfected with vector, si-EN2, or si-EN2 + CCL20, respectively. b, c The capacity of cell proliferation determined by CCK8 assay (b) and cell clone-formation assay (c). d The capacity of cell migration assessed by transwell assay. Scale bar: 100 µm. e The capacity of cell migration assessed by wound healing test. Scale bar: 0.5 mm. Data are presented as mean ± SEM from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001.
