Fig. 4: FXR suppresses the activity of Wnt/β-catenin signaling in colorectal tumorigenesis. | Cell Death & Disease

Fig. 4: FXR suppresses the activity of Wnt/β-catenin signaling in colorectal tumorigenesis.

From: Farnesoid X receptor antagonizes Wnt/β-catenin signaling in colorectal tumorigenesis

Fig. 4

a Hierarchical clustering of genes that were significantly and differentially expressed in HT-29-shFXR cells and control cells. Data were log2 normalized. b Pathway analysis of genes that were significantly and differentially expressed in HT-29-shFXR cells and control cells using KEGG database. c The effect of FXR knockdown or overexpression on the luciferase activities of TOP/FOP-Flash reporter plasmid. d The effect of FXR knockdown or overexpression on the mRNA levels of cyclin D1 and c-Myc in colon cancer cells detected by real-time PCR. e, f The effect of FXR knockdown (e) or overexpression (f) on the protein levels of cyclin D1 and c-Myc in colon cancer cells detected by western blotting analysis. All data are the mean ± SD of three independent experiments. *P < 0.05.

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