Fig. 2: lnc-AC104041.1 acts as an oncogenic driver for HNSCC cells. | Cell Death & Disease

Fig. 2: lnc-AC104041.1 acts as an oncogenic driver for HNSCC cells.

From: Antisense oligonucleotides targeting lncRNA AC104041.1 induces antitumor activity through Wnt2B/β-catenin pathway in head and neck squamous cell carcinomas

Fig. 2

a qRT-PCR analysis of AC104041.1 in HNSCC cells and normal epithelial cell. Data are mean values ± SD, the experiment was performed in triplicates and repeated three times, ***P < 0.001 (Student’s t-test). b The proliferation ability was compared in four different HNSCC cells (n = 5). Data are mean values ± SEM, the experiment was repeated three times, *P < 0.05, **P < 0.01, ***P < 0.001 (two-way ANOVA). c The migration ability was compared in four different HNSCC cells. Data are mean values ± SD, the experiment was performed in triplicates and repeated three times, ***P < 0.001 (Student’s t-test). MTT proliferation assay of SCC4 cells with AC104041.1 knockdown (d) or CAL27 cells with AC104041.1 overexpression (e). n = 5, the experiment was repeated three times. Data are presented as the mean values ± SEM, ***P < 0.001, compared with control cells (two-way ANOVA). Migration assay in SCC4 cells with knockdown (f) or CAL27 cells with overexpression (g) of AC104041.1. Data are presented as the mean values ± SD, the experiment was performed in triplicates and repeated three times, ***P < 0.001 (Student’s t-test). Tumor growth in Balb/c nude mice 8 weeks after subcutaneous inoculation of SCC4 cells with AC104041.1 knockdown (h) or CAL27 cells with AC104041.1 overexpression (i), n = 5 mice for each group. Data are presented as the mean values ± SEM, ***P < 0.001 (two-way ANOVA). Bioluminescence signal of SCC4 cells with AC104041.1 knockdown (j) or CAL27 cells with AC104041.1 overexpression (k) detected using an in vivo imaging system for 8 weeks (n = 5 mice for each group). Data are presented as the mean values ± SD, ***P < 0.001 (two-way ANOVA).

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