Fig. 6: Salinomycin promotes the anti-tumor activity of AC104041.1 specific LNA-ASO in vitro and in vivo. | Cell Death & Disease

Fig. 6: Salinomycin promotes the anti-tumor activity of AC104041.1 specific LNA-ASO in vitro and in vivo.

From: Antisense oligonucleotides targeting lncRNA AC104041.1 induces antitumor activity through Wnt2B/β-catenin pathway in head and neck squamous cell carcinomas

Fig. 6

a Cell proliferation assay of SCC4 cells transfected with AC104041.1 specific LNA-ASO or combined with salinomycin treatment (n = 5). Data are presented as the mean values ± SEM, the experiment was repeated three times, ***P < 0.001, compared with control cells (two-way ANOVA). b Cell migration assay of SCC4 cells transfected with AC104041.1 specific LNA-ASO or combined with salinomycin treatment. Data are presented as the mean values ± SD, the experiment was performed in triplicates and repeated three times, **P < 0.01, ***P < 0.001 (Student’s t-test). c Tumor growth in Balb/c nude mice and representative immunohistochemical images of Ki-67 from tumors after subcutaneous injection of AC104041.1 specific LNA-ASO or combined with salinomycin treatment (n = 5 mice for each group). Data are presented as the mean values ± SEM, **P < 0.01, ***P < 0.001 (two-way ANOVA). d Immunofluorescence of β-catenin (green) and nuclei (blue) in SCC4 cells transfected with AC104041.1 specific LNA-ASO or combined with salinomycin treatment. Scale bar represents 20 µm.

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