Fig. 1: miR-20b targets ULK1 and regulates its expression. | Cell Death & Disease

Fig. 1: miR-20b targets ULK1 and regulates its expression.

From: Propofol-induced MiR-20b expression initiates endogenous cellular signal changes mitigating hypoxia/re-oxygenation-induced endothelial autophagy in vitro

Fig. 1

a Luciferase reporter constructs. The 3′UTR (1–1801 bp) of ULK1 was inserted downstream of the firefly luciferase gene of the pmirGLO vector, named pmirGLO-ULK1. MRE denotes miRNA response element. b Luciferase reporter assay of the interaction between miR-20b and the predicted MRE in HUVECs. Each pmirGLO-ULK1 was co-transfected with negative control (NC), miR-20b mimics (20b), inhibitor NC (IN NC), or miR-20b inhibitor (IN 20b) into HUVECs. Detection of the luciferase activity was done after 24 h. The firefly luciferase activity was normalized to Renilla. c, d qPCR was performed to detect the expression of miR-20b and ULK1 in HUVECs, respectively. e, f HUVECs lysates were prepared and subjected to western blot analysis by using anti-ULK1 antibody. Data were mean ± SEM (n = 3); *p < 0.05,**p < 0.01, ***p < 0.001.

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