Fig. 3: miR-20b regulates H/R-induced autophagy by targeting ULK1. | Cell Death & Disease

Fig. 3: miR-20b regulates H/R-induced autophagy by targeting ULK1.

From: Propofol-induced MiR-20b expression initiates endogenous cellular signal changes mitigating hypoxia/re-oxygenation-induced endothelial autophagy in vitro

Fig. 3

a The HUVECs were transfected with negative control (NC), miR-20b mimics (20b), inhibitor NC (IN NC), miR-20b inhibitor (IN 20b) for 24 h. Then the HUVECs in hypoxic conditions were placed as above for 12 h. The medium was only replaced and placed in normal condition for 4 h after hypoxia. b–f Total proteins were extracted to detect ULK1, P62, Beclin1, LC3II/I (16KD/18KD) by using β-actin as a reference. Densitometric ratios of these proteins were quantified by using IMAGEJ. g Samples were stained with anti-LC3 (red) and anti-p62 (blue) antibodies. Bar, 10 μm. h Samples were stained with ULK1 (red) antibodies. Bar, 10 μm. i Electron microscopy was used to assess the inhibitory role of miR-20b in the H/R-induced autophagy. Bar, 1 μm. Data are mean ± SEM (n = 3); *p < 0.05, **p < 0.01, ***p < 0.001.

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