Fig. 4: Re-expression of ULK1 restores autophagy inhibited by miR-20b. | Cell Death & Disease

Fig. 4: Re-expression of ULK1 restores autophagy inhibited by miR-20b.

From: Propofol-induced MiR-20b expression initiates endogenous cellular signal changes mitigating hypoxia/re-oxygenation-induced endothelial autophagy in vitro

Fig. 4

Built the H/R model as above. a–d HUVECs were transfected with negative control (NC) and miR-20b mimics (20b) or co-transfected with miR-20b mimics (20b) and the constructs (ULK1-Flag) were indicated for 24 h. Using a normal group as a control. Built the H/R model with these cells. Protein samples were collected and the expression of ULK1, Flag, LC3 (16KD/18KD), and P62 was detected through western blot. β-actin was used as a reference. e Treat the cells as in a. Then the cells were processed for immunofluorescence with anti-TOM20 (red) and LC3 (green) antibodies. Bar, 10 μm. Data are mean ± SEM (n = 3); *p < 0.05, **p < 0.01,***p < 0.001.

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