Fig. 2: ARHGEF16 promotes colorectal cancer cell proliferation.
From: FYN is required for ARHGEF16 to promote proliferation and migration in colon cancer cells
a HCT116 cells were transfected with Vector or Lv-ARHGEF16 for 48 h and harvested for WB analysis with the indicated antibodies. b ARHGEF16 overexpression increased the colony formation ability of HCT116 cells. c Quantification of the colony formation rates was shown in Fig. 2b. Data are shown as the mean ± SD (n = 5). P-values were obtained by the two-side Student’s t test. **P < 0.01. d ARHGEF16 overexpression increased the migration of HCT116 cells. e Quantification of the migration rates was shown in Fig. 2d. Data are shown as the mean ± SD (n = 5). **P < 0.01. P-values were obtained by the two-side Student’s t test. f ARHGEF16 overexpression increased the invasion of HCT116 cells. g Quantification of the invasion rates was shown in Fig. 2f. Data are shown as the mean ± SD (n = 5). **P < 0.01. P-values were obtained by the two-side Student’s t test. h SW620 cells were transfected with Sh-control or Sh-ARHGEF16 #1 and harvested for WB analysis with the indicated antibodies. i Knockdown of ARHGEF16 decreased the colony formation ability of SW620 cells. SW620 cells were transfected with Sh-control or Sh-ARHGEF16 #1 and Sh-ARHGEF16 #2. P-values were obtained by the two-side Student’s t test. Data are shown as the mean ± SD (n = 5). *P < 0.05. j ARHGEF16 knockdown with Sh-control or Sh-ARHGEF16 #1 and Sh-ARHGEF16 #2 decreased the proliferation rate of SW620 cells, as shown by EdU staining. P-values were obtained by the two-side Student’s t test. Data are shown as the mean ± SD (n = 5). *P < 0.05. k ARHGEF16 knockdown with Sh-control or Sh-ARHGEF16 #1 decreased the colony formation ability of HT29 cells. **P < 0.01. P-values were obtained by the two-side Student’s t test. Data are shown as the mean ± SD (n = 5).
