Fig. 1: pY772-EphA2 is responsible for EphA2-dependent NPC cells growth in vitro and in vivo. | Cell Death & Disease

Fig. 1: pY772-EphA2 is responsible for EphA2-dependent NPC cells growth in vitro and in vivo.

From: Y772 phosphorylation of EphA2 is responsible for EphA2-dependent NPC nasopharyngeal carcinoma growth by Shp2/Erk-1/2 signaling pathway

Fig. 1

a Establishment of 5-8F and CNE2 cell lines with the stable expression of exogenous EphA2 (EphA2-WT) or EphA2-Y772A (EphA2-YA) using endogenous EphA2-knockdown (shEphA2) cells. CCK-8 (b), EdU incorporation (c), and plate clone formation (d) assay showing the proliferation of NPC cells expressing EphA2-WT or EphA2-YA and their control cells. e Soft agar colony formation assay showing the anchorage-independent growth of NPC cells expressing EphA2-WT or EphA2-YA and their control cells. f, g Subcutaneous tumor formation experiment showing the growth of NPC cells expressing EphA2-WT or EphA2-YA and their control cells. The images of xenografts after 21 days subcutaneous implantation of the cells (f). Growth and weight of the xenograft tumors (g). n = 5 mice per group. Numbers represent mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., no significance.

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