Fig. 3: pY772-EphA2 mediates EphA2-activating Shp2/Erk-1/2 signaling pathway in the NPC cells. | Cell Death & Disease

Fig. 3: pY772-EphA2 mediates EphA2-activating Shp2/Erk-1/2 signaling pathway in the NPC cells.

From: Y772 phosphorylation of EphA2 is responsible for EphA2-dependent NPC nasopharyngeal carcinoma growth by Shp2/Erk-1/2 signaling pathway

Fig. 3

a Western blotting showing the levels of p-Shp2 and p-Erk-1/2 in the NPC cells expressing EphA2-WT, EphA2-YA, or EphA2-SA and their control cells. b Western blotting showing the levels of p-Erk-1/2 in the EphA2-YA-expressed NPC cells transfected with Shp2 expression plasmid and their control cells. c Western blotting showing the levels of p-Erk-1/2 in the EphA2-WT-expressed NPC cells infected with the lentiviral vector expressing Shp2 shRNA. d Western blotting showing the levels of p-Erk-1/2 in the EphA2-WT-expressed NPC cells treated with Shp2 inhibitor SHP099 for 12 h. e Western blotting showing the levels of p-Shp2 and p-Erk-1/2 in the xenografts of NPC cells expressing EphA2-WT or EphA2-YA and their control cells. f Immunohistochemistry (IHC) showing the levels of p-Shp2 and p-Erk-1/2 in the xenografts of NPC cells expressing EphA2-WT or EphA2-YA and their control cells. Representative IHC images are shown on the left and statistical analysis is presented on the right. Scale bar = 50 μm. Numbers represent mean ± SD. ***P < 0.001; no, no significance. OE overexpression; shCtrl, scramble non-target RNA.

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