Fig. 4: pY772-EphA2 promotes NPC cell proliferation and anchorage-independent growth by Shp2 signaling. | Cell Death & Disease

Fig. 4: pY772-EphA2 promotes NPC cell proliferation and anchorage-independent growth by Shp2 signaling.

From: Y772 phosphorylation of EphA2 is responsible for EphA2-dependent NPC nasopharyngeal carcinoma growth by Shp2/Erk-1/2 signaling pathway

Fig. 4

CCK-8 (a), EdU incorporation (b), and plate clone formation (c) assay showing the proliferation of EphA2-YA-expressed NPC cells transfected with Shp2 expression plasmid, EphA2-WT-expressed NPC cells infected with the lentiviral vector expressing Shp2 shRNA, and their control cells. d Soft agar colony formation assay showing the anchorage-independent growth of EphA2-YA-expressed NPC cells transfected with Shp2 expression plasmid, EphA2-WT-expressed NPC cells infected with the lentiviral vector expressing Shp2 shRNA, and their control cells. e Shp2 inhibitor SHP099 inhibits EphA2-WT-expressed NPC cell proliferation. Cells were treated with 1, 2, or 5 μm SHP099 for 72 h and cell proliferation was assayed by CCK-8. Cell proliferation in SHP099 treatment group relative to a DMSO control group is shown. Numbers represent mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; n.s., no significance.

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