Fig. 4: GADD45β interacts with MEKK4 in response to ALV-J infection-induced activation of the p38MAPK signaling pathway. | Cell Death & Disease

Fig. 4: GADD45β interacts with MEKK4 in response to ALV-J infection-induced activation of the p38MAPK signaling pathway.

From: ALV-J inhibits autophagy through the GADD45β/MEKK4/P38MAPK signaling pathway and mediates apoptosis following autophagy

Fig. 4

a DF-1 cells or CEF cells were transfected with siRNA targeting GADD45β for 24 h and subsequently infected with ALV-J for 24 h. The infected cells were then collected for western blotting analysis of the expression levels of gp37, p-p38, p38, p-JNK, JNK, p-ERK, ERK, LC3B, SQSTM1, and Atg5. b DF-1 cells were co-transfected with Flag-GADD45β and HA-MEKK4 for 24 h and the colocalization of GADD45β (red) with MEKK4 (green) was detected using the corresponding antibodies under confocal microscopy. Scale bar, 10 μm. c DF-1 cells were co-transfected with Flag-GADD45β and HA-MEKK4 for 48 h, and the interaction between GADD45β and MEKK4 was determined using the indicated antibodies. d ALV-J infection was performed following the transfection with siRNA targeting MEKK4 in DF-1 cells or CEF cells for 24 h. Expression levels gp37, p-p38, p38, p-JNK, JNK, LC3B, SQSTM1, and Atg5 were analyzed using western blotting. Data are presented as the mean ± SD of three independent experimental repeats.

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